Program Overview

Genomics and Next Generation Sequencing

Course Type

The course type is Postgraduate.

Course Details

This module will introduce students to various aspects of *Omics, including:

• Genomics
• Transcriptomics
• Methylation
• Transcription factors analysis
• RNA binding protein analysis
• Chromatin accessibility analysis (e.g., DNase-seq, ATAC-seq)
• Chromatin structure analysis (e.g., HiC, ChIA-PET)

It will cover technological progress, including:

• History: Sanger sequencing through array technologies
• Next generation Sequencing
• Advanced library construction procedures for specialized assays (e.g., ChIP, DNase, ATAC, HiC, eCLIP)

The module will also address specific fields of:

• Classical Genetics
• Population Genetics
• Cancer Genomics

It will involve a biological, technological, and analytical dimension to help students design the best experiment with the appropriate data type and enable its analysis with the latest state-of-the-art approaches.

By the end of the module, students should be able to:

• Understand the biological interpretation of the various *omics fields, especially DNA, RNA, and Methylation-based.
• Understand the various technologies available to measure the different types of information from Sanger sequencing, micro-array, Mass-Spectrometry to Next Generation sequencing.
• Analyze the various types of data generated in the field, both with command line and web interface, such as Galaxy.
• Integrate the various types of data to understand the biological implications of the results.
• Deal with the complexity of information available to enable the integration of diverse data types.

Assessment

The module is assessed by a combination of a written examination (60%) in the form of short answer questions and a group presentation (40%) during the course of the module. There will also be a formative written examination in the form of MCQ during this module.

Module Lead

The module lead is Professor Jean-Baptiste Cazier.

Availability

Please note that the module is only available as part of MSc Bioinformatics and the International Doctoral Training Programme.