Hands-On Introduction to Preparative Chromatography
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| Program start date | Application deadline |
| 2026-10-19 | - |
| 2027-10-19 | - |
Program Overview
Hands-On Introduction to Preparative Chromatography
This 2.5-day course introduces key concepts in downstream processing with a focus on protein purification at laboratory scale using chromatographic techniques. Participants will gain both theoretical insights and hands-on experience in purifying antibody fragments derived from camelids that are widely used in pharmaceutical diagnostics and therapy.
Course Schedule
- Day 1 (08:30-16:00)
- Lecture: Course orientation
- Lecture: From upstream to downstream: an overview
- Lecture and workshop: Resins and column packing
- Lecture and workshop: Classes of chromatography
- Lecture: Introduction to lab work
- Lab: Buffer Preparation and chromatography workstation software
- Day 2 (08:30-16:00)
- Lab: Preparative ion exchange chromatography (IEX)
- Lab: Preparative affinity chromatography (IMAC)
- Lab: Data collection and discussion
- Lab: Analytical size exclusion chromatography (SEC) and gel electrophoresis (SDS-PAGE)
- Lecture: Scale-up (industry speaker)
- Day 3 (09:00-13:30)
- Lab: Data analysis and system clean up
- Discussion
- Groupwork and presentations
Learning Outcomes
At the end of this course, participants will be able to:
- Gain a broad understanding of downstream processing and explain the principles and applications of multiple types of chromatography in protein purification.
- Design a stepwise protein purification protocol based on physicochemical properties and process requirements.
- Interpret chromatograms to evaluate purification efficiency, identify target peaks, and estimate yield and purity.
- Troubleshoot common issues in chromatographic protein purification using systematic problem-solving approaches.
- Evaluate the scalability of purification processes and understand relevant regulatory considerations in the pharmaceutical industry.
Target Audience
This course is broadly applicable for professionals in the biotechnology, pharmaceutical, and biopharma industries who are involved in the development, production, or quality control of biologics. It is especially relevant for:
- Scientists and technicians working with protein purification or chromatography
- Process engineers involved in downstream development or scale-up
- QA/QC and regulatory affairs professionals seeking insight into purification workflows
- Researchers and academic staff transitioning to industrial applications
- Early career professionals looking to build core competencies in downstream processing
- Experienced staff wanting to refresh and update their knowledge with current best practices
Prerequisites
Participants do not need to have specific knowledge in the field of biotechnology other than a basic high-school level understanding of natural sciences.
Course Details
- Duration: 3 days
- Place: DTU Lyngby Campus
- Price: 20,000.00 DKK
- Variant: Choose an option (2026 October 19th-21th)
Related Educations
- Fundamentals of Microbial Fermentation Design
- Introduction to Biomanufacturing
- Hands-On Introduction to Mammalian Bioprocessing
- Process Analytical Technology
Course Responsible
Esperanza Rivera de Torre, Assistant Professor, Department of Biotechnology and Biomedicine, Section for Biologics Engineering and Antibody Technologies.