Program Overview

Genomics Core Program

The Genomics Core program offers a range of services, supplies, and instrumentation for comparative genomics. The program provides training and support for researchers in various molecular biology techniques and software.

Services

The Genomics Core offers the following services:

• DNA sequencing and fragment analysis
• Quantitative and real-time PCR
• DNA size-selection and quantitation
• Library construction for next-generation sequencing
• Bioinformatics support and data analysis

Training Options

The program provides various training options, including:

• Self-paced online training
• In-house training
• Vendor training

In-House Training

The Genomics Core offers in-house training in the following areas:

• Project development
• Sanger sequencing
• Sequence Scanner software
• qPCR software
• DNA quantitation methods
• DNA size-selection methods
• PCR basics
• Restriction digest basics
• Online GC submissions and Biosci-Booked (Genomics)

Vendor Training

The program provides vendor training in the following areas:

• Fundamentals of real-time PCR
• qPCR assay design choices
• qPCR normalization
• qPCR multiplexing
• qPCR reagent choices
• qPCR theory of analysis
• Protein thermal shift
• 3130xl special purpose assays
• 3130xl GeneMapper software
• 3130xl Minor Variant Finder software
• 3130xl SeqScape software
• 3130xl cloud analysis
• Ion NGS microbial applications
• Ion NGS RNA applications
• Ion NGS targeted applications
• Ion NGS library construction

Equipment and Protocols

The Genomics Core has the following equipment and protocols:

• Ion Torrent S5 (NGS)
• ABI 3130xl (DNA sequencing/fragment analysis)
• ABI (qPCR)
• Diagenode Bioruptor NGS
• Agilent Bioanalyzer
• E-gel (DNA size-selection)
• BluePippin (DNA size selection for NGS)
• SpeedVac SPD1010
• Typhoon 8600 Variable Mode Imager
• Eppendorf Centrifuge 5810R (plates, 15ml tubes, 50ml tubes)
• Eppendorf Centrifuge 5425R (24-place rotor, 1.5ml tubes)
• Qubit 4.0 (DNA/RNA/Protein quantitation; RNA integrity)
• Nanodrop (nucleic acid and protein quantitation)